A Simple Eco-Friendly Stability-Indicating HPLC Method for Determination of NETILMICIN in Bulk and Ophthalmic Solution.

The aim of our study was the development and validation of an eco-friendly stability-indicating method for the determination of netilmicin in bulk and in ophthalmic solution. The mobile phase was composed of 40-mL ethanol and 960-mL buffer solution at a pH of 3.5, which was prepared by using 1-hexane sodium sulfonate and anhydrous sodium sulfate, at a flow rate of 1 mL/min. The detecting wavelength was 205 nm, the injection volume was 20 μL, the analysis runtime was < 7 min, and the focus was on netilmicin concentration levels of 125.0-1000.0 μg/mL. The method's environmental friendliness was demonstrated by the conclusional AES of 87, the AGREE of 0.65, the MoGAPI of 76 with six green pieces, the BAGI score of 77.5, and the whiteness of 99.2. The method that is being provided demonstrates exceptional attributes including cost-effectiveness, ICH validation items, and environmental friendliness.