Blood · 2021
Inherited glycosylphosphatidylinositol defects cause the rare Emm-negative blood phenotype and developmental disorders.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Duval Romain, Nicolas Gaël, Willemetz Alexandra, Murakami Yoshiko, Mikdar Mahmoud, Vrignaud Cedric, Megahed Hisham, Cartron Jean-Pierre, Masson Cecile, Wehbi Samer, Koehl Bérengere, Hully Marie, Siquier Karine, Chemlay Nicole, Rotig Agnes, Lyonnet Stanislas, Colin Yves, Barcia Giulia, Cantagrel Vincent, Le Van Kim Caroline, Hermine Olivier, Kinoshita Taroh, Peyrard Thierry, Azouzi Slim
Аннотация
Glycosylphosphatidylinositol (GPI) is a glycolipid that anchors >150 proteins to the cell surface. Pathogenic variants in several genes that participate in GPI biosynthesis cause inherited GPI deficiency disorders. Here, we reported that homozygous null alleles of PIGG, a gene involved in GPI modification, are responsible for the rare Emm-negative blood phenotype. Using a panel of K562 cells defective in both the GPI-transamidase and GPI remodeling pathways, we show that the Emm antigen, whose molecular basis has remained unknown for decades, is carried only by free GPI and that its epitope is composed of the second and third ethanolamine of the GPI backbone. Importantly, we show that the decrease in Emm expression in several inherited GPI deficiency patients is indicative of GPI defects. Overall, our findings establish Emm as a novel blood group system, and they have important implications for understanding the biological function of human free GPI.