Contraception · 2008
A possible role of progesterone receptor in mouse oocyte in vitro fertilization regulated by norethisterone and its reduced metabolite.
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Flores-Herrera Héctor, Díaz-Cervantes Paola, De la Mora Gustavo, Zaga-Clavellina Verónica, Uribe-Salas Felipe, Castro Ivone
Аннотация
BACKGROUND: The contraceptive effect of the progestogen norethisterone (NET) and its main metabolites 5alpha-NET and 3beta,5alpha-NET has been demonstrated in several species, and most studies have focused on the effects of these compounds in the uterus. We previously reported that 5alpha-NET inhibits the progesterone (P(4))-induced acrosome reaction in pig and mouse spermatozoa and induces severe morphological damage in two-cell fertilized mouse oocytes.
STUDY DESIGN: The main goal of this study was to analyze the possible role of P(4) receptor (PR) in the effects of NET and 5alpha-NET on the oocyte fertilization process. Different steroid treatments were used with or without cumulus-enclosed oocytes.
RESULTS: It was demonstrated that NET increases the percentage of fertilized oocytes in the same manner as P(4) does, while 5alpha-NET reduces the percentage of fertilized oocytes. This effect was not reversed by P(4) in the same concentrations.
CONCLUSION: A possible molecular mechanism for the effects of 5alpha-NET may be through a PR localized in the oocyte plasma membrane.
STUDY DESIGN: The main goal of this study was to analyze the possible role of P(4) receptor (PR) in the effects of NET and 5alpha-NET on the oocyte fertilization process. Different steroid treatments were used with or without cumulus-enclosed oocytes.
RESULTS: It was demonstrated that NET increases the percentage of fertilized oocytes in the same manner as P(4) does, while 5alpha-NET reduces the percentage of fertilized oocytes. This effect was not reversed by P(4) in the same concentrations.
CONCLUSION: A possible molecular mechanism for the effects of 5alpha-NET may be through a PR localized in the oocyte plasma membrane.