Flight energetics, caste dimorphism and scaling properties in the bumblebee, Bombus impatiens.

Animal size affects the energetics of locomotion. Using female caste dimorphism in bumblebees, we assessed how body mass impacts morphological and physiological traits linked with flight. The allometric relationships obtained for wing surface area, wingbeat frequency, and flight and resting metabolic rates of workers could predict the trait values of queens that were more than fourfold larger. Flight success of queens decreased over time in part because of a large increase in body mass and a decrease in traits linked with flight, namely wingbeat frequency and metabolic rate, and the activity of metabolic enzymes tended to decrease. After taking into account temporal changes, body mass, flight wingbeat frequency and metabolic rate were repeatable. Finally, we found significant family resemblance for all traits measured, indicating that shared genes and/or environmental effects impact phenotypic variation. Together, our results show that the functional association between body morphology and flight physiology is robust, providing further insights into the mechanistic basis of metabolic rate scaling patterns during locomotion in animals.