[A simple gas-chromatographic method for the determination of 2,2-diethylallylacetamide in serum and its application in cases of poisoning (author's transl)].

A simple gas-chromatographic method for the determination of 2,2-diethylallylacetamide is described. A saturated solution of ammonium sulfate is added to 1 ml serum and the mixture is extracted by chloroform. The organic phase is evaporated. The residue is dissolved in ethylacetate/acetic acid and injected into gas-chromatograph. The coefficient of variation for the precision in the series is 8,5%, the recovery 76%, the detection limit 3,2 mumol/l. The extract can be used for the determination of other hypnotics, e.g. barbiturates or carbromal. In cases of poisoning the concentration of 2,2-diethylallylacetamide exceeded 700 mumol/l.