New 4-Methoxyglucosides With Anti-A549 Activity From Bidirectional Solid Fermentation of Scutellaria barbata and Cordyceps kyushuensis.

The fermentation products were obtained through bidirectional solid fermentation technology, utilizing Scutellaria barbata D. Don as the medicinal substrate and Cordyceps kyushuensis as the fermentation strain. Five novel 4-methoxyglucosides, one new non-nucleoside cyclic peptide and 36 known compounds were isolated from the ethanol extract of the fermentation products. Notably, in the five novel glucosides, the 4-position hydroxyl group was modified to a methoxy group, likely due to enzymatic reactions during fermentation. In vitro assays revealed that compounds 4, 5, 16, 19, 20, 25, 27, 31, and 37 exhibited inhibitory effects on A549 cells, with the IC50 values for the known compound 31 being 38.5 µM.