Construction of Hyaluronic Acid-CeO₂ Conjugated Composite Nanoparticles and Their Activity Efficiency in Diabetic Retinopathy Alleviation.

We developed an effective nanoparticle-biomaterial in alleviating diabetic retinopathy (DR), hyaluronic acid (HA)-CeO₂, composed mainly of CeO₂ and HA. To demonstrate its anti-DR capacity, retinal cells from a B6/J mouse model were used to compare the efficiency of PEI-CeO₂ and HA-CeO₂. We investigated the transport performance, histolysis, immune cell infiltration, angiogenesis, and hyperemia induced by the transport system. The structural integrity, microvascular apoptosis, and superoxide and peroxide concentrations in the retina were measured to evaluate the clinical efficacy of CeO₂. The infiltration efficiency of HA-CeO₂ was higher than that of PEI-CeO₂. Lower levels of foreign body reaction were evident for HA-CeO₂ with less histolysis, immune cell infiltration, angiogenesis, and hyperemia. The clinical efficacy of HA-CeO₂ in terms of preservation of retinal structure and lowering of microvascular apoptosis and superoxide and peroxide concentrations was superior to those of PEI-CP. HA-CeO₂ was shown to have significant antioxidation and anti-vascular injury capacity in a mouse model, and may be a potential compound nanodrug for DR treatment in the future.