Journal of B.U.ON. : official journal of the Balkan Union of Oncology · 2016
Thymidylate synthase polymorphism in Mexican patients with colon cancer treated with 5-fluorouracil.
СтатьяНазвание и аннотация: Перевод в очереди
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Patricia Rios-Ibarra Clara, Janeth Rodriguez-Silva Claudia, Alonso Lopez-Chuken Yamil, Ortiz-Lopez Rocio, Fernandez-Castillo Elisa, Beatriz Del Toro Runzer Claudia, Paul Armenta-Perez Vicente, Flores-Gutierrez Juan Pablo, Quintanilla-Guzman Arturo, Salinas-Santander Mauricio, Gonzalez-Guerrero Juan Francisco, Bosques-Padilla Francisco, Santos Arturo, Guadalupe Martinez-Rodriguez Herminia
Аннотация
PURPOSE: We analyzed the genotype and allele frequency of variable number tandem repeats (VNTR)-thymidylate synthase (TS) and its relationship with the disease evolution in colon cancer patients.
METHODS: We selected 24 paraffin-embedded colon cancer tissue samples from Mexican patients who received a 5-fluorouracil (5-FU)-based chemotherapy regimen. Tumor tissue was digested with proteinase K and genomic DNA was isolated by the standard method with phenol-chloroform extraction. Polymerase chain reaction (PCR) was performed for TS genotyping of VNTR and the results were evaluated directly in a stained agarose gel.
RESULTS: The allele frequency of 2 repeats (2R) was greater (0.66) than 3R (0.34) in metastatic colon cancer (x2=10.24; p=0.001)) however, no difference in allelic distribution between 2R (0.54) and 3R (0.46) in non metastatic patients was observed (x2=0.640; p=0.424).
CONCLUSION: Our results suggest that Mexican patients with colon cancer present differences in the allelic distribution, the 2R allele being the most frequent.
METHODS: We selected 24 paraffin-embedded colon cancer tissue samples from Mexican patients who received a 5-fluorouracil (5-FU)-based chemotherapy regimen. Tumor tissue was digested with proteinase K and genomic DNA was isolated by the standard method with phenol-chloroform extraction. Polymerase chain reaction (PCR) was performed for TS genotyping of VNTR and the results were evaluated directly in a stained agarose gel.
RESULTS: The allele frequency of 2 repeats (2R) was greater (0.66) than 3R (0.34) in metastatic colon cancer (x2=10.24; p=0.001)) however, no difference in allelic distribution between 2R (0.54) and 3R (0.46) in non metastatic patients was observed (x2=0.640; p=0.424).
CONCLUSION: Our results suggest that Mexican patients with colon cancer present differences in the allelic distribution, the 2R allele being the most frequent.