Enriching Mn-Doped ZnSe Quantum Dots onto Mesoporous Silica Nanoparticles for Enhanced Fluorescence/Magnetic Resonance Imaging Dual-Modal Bio-Imaging.

Multimodal imaging is more suitable for disease diagnosis because of its ability to provide more complementary and accurate information over single-mode imaging. Mn-doped quantum dots (QDs), especially Mn-doped ZnS (ZnSe) QDs, possess unique fluorescent and magnetic properties and are thus attractive for fluorescence/magnetic resonance imaging (MRI) dual-mode imaging. However, the optimal dopant (Mn2+) concentration for maximizing the fluorescence of QDs is relatively low for the MRI imaging. Herein, based on the large Stokes shift of Mn-doped ZnSe QDs, an enrichment strategy with mesoporous silica (MSN) loading was explored for constructing a highly luminescent/paramagnetism Mn-doped ZnSe QDs assembly (MSN@QDs) for improved MRI/optical dual-model imaging. After assembly, the loading density of QDs in MSNs was estimated to be 152 ± 12. Upon loading, the fluorescence of the MSN@QDs assembly was enriched along with QDs (enrichment factor of ∼143). Because of the large Stokes shift (∼200 nm), no appreciable concentration quenching was observed. Meanwhile, the T1 MR contrast was also increased both in vitro and in vivo through improved local Mn2+ concentration, realizing MRI signal enrichment. In fluorescence imaging investigations, MSN@QDs showed better performance over both single QDs and equivalent numbers of MSN-free single QD. Therefore, this enrichment strategy allowed simultaneous signal enhancement of the two imaging modes of Mn-doped ZnSe QDs.