Title: Impact of CYP3A5*3 genetic polymorphism on breast cancer risk: Evidence from Bangladesh.

BACKGROUND

The CYP3A5 gene plays a crucial role in drug metabolism and carcinogen activation, potentially influencing breast cancer susceptibility. However, its genetic association with breast cancer risk in the Bangladeshi population remains under explored.

OBJECTIVE

This study aims to examine the expression and genetic correlation of the CYP3A5*3 (rs776746) polymorphism in relation to breast cancer susceptibility.

METHODS

A case-control study was performed with 150 breast cancer patients and 150 healthy controls to investigate genotypic variations of the CYP3A5*3 (rs776746) polymorphism using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. The relationship between the CYP3A5*3 variant and breast cancer risk was assessed by calculating odds ratios (ORs) and 95% confidence intervals (CIs) through logistic regression models.

RESULTS

The CYP3A5*3 polymorphism exhibited a significant association with increased breast cancer risks. Breast cancer risk was found to be higher in heterozygotes than in homozygotes. Individuals carrying the heterozygous (*1/*3) and mutant homozygote (*3/*3) genotypes had a 3.04-fold (p = 0.0005) and 1.9-fold (p = 0.0227) increased risk of developing breast cancer respectively. Furthermore, the combined (*1/*3 + *3/*3) genotype was significantly linked to a 3.67-fold higher susceptibility to breast cancer risks (p < 0.0005).

CONCLUSION

Our findings suggest that the CYP3A5*3 polymorphism is significantly associated with an elevated risk of breast cancer in Bangladeshi population.