Wei sheng yan jiu = Journal of hygiene research · 2010
[Analysis on opioid compounds in the hair of heroin addicts with LC-MS/MS].
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhang Jianxin, Meng Pinjia, Zhang Daming, Zhang Wenfang, Chen Cunyi
Аннотация
OBJECTIVE: To evaluate the effects of washing process on hair samples from heroin addicts, and compare the extracting method of enzymatic digestion with homogenization of hair samples.
METHODS: After washing samples for 3.15 h, a mild enzymatic digestion procedure at pH 6.6 and a buffer incubating procedure in water bath at 48 degrees C during 18h was used. The samples were pre-treated by solid phase extraction using mixed mode sorbent columns (MCX Oasis). Quantitative analysis of morphine, codeine, 6-acetylmorphine, heroin, acetylcodeine by chromatography-tandem mass spectrometry method was developed.
RESULTS: Heroin accounted for 21.82% of the total equivalents of morphine. The ratio of morphine to 6-MAM in enzymatic digestion hair samples was 0.9875 and that in homogenized hair samples was 0.3948.
CONCLUSION: The results suggested that the procedures were not sufficient to remove the contaminants penetrating into hair from external sources.
METHODS: After washing samples for 3.15 h, a mild enzymatic digestion procedure at pH 6.6 and a buffer incubating procedure in water bath at 48 degrees C during 18h was used. The samples were pre-treated by solid phase extraction using mixed mode sorbent columns (MCX Oasis). Quantitative analysis of morphine, codeine, 6-acetylmorphine, heroin, acetylcodeine by chromatography-tandem mass spectrometry method was developed.
RESULTS: Heroin accounted for 21.82% of the total equivalents of morphine. The ratio of morphine to 6-MAM in enzymatic digestion hair samples was 0.9875 and that in homogenized hair samples was 0.3948.
CONCLUSION: The results suggested that the procedures were not sufficient to remove the contaminants penetrating into hair from external sources.