PloS one · 2020
The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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So Seongjun, Lee Yeonmi, Choi Jiwan, Kang Seoon, Lee Ji-Yoon, Hwang Julie, Shin Joosung, Dutton James R, Seo Eul-Ju, Lee Beom Hee, Kim Chong Jai, Mitalipov Shoukhrat, Oh Soo Jin, Kang Eunju
Аннотация
Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.
Предметные рубрики MeSH
1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAmidesCell DifferentiationCell LineCell ProliferationCell SurvivalEmbryonic Stem CellsHumansInduced Pluripotent Stem CellsNeural CrestPluripotent Stem CellsProtein Kinase InhibitorsPyridinesRetinal Pigment EpitheliumStem Cell Researchrho-Associated Kinases