Streptomycin interference in Jaffe reaction - possible false positive creatinine estimation in excessive dose exposure.

OBJECTIVES

To study the potential of commonly used aminoglycoside antibiotics to form non-creatinine chromogen with alkaline picrate reagent.

DESIGN AND

METHODS

We studied the non-creatinine chromogen formation of various concentrations of streptomycin, amikacin, kanamycin, netilmicin, gentamicin and tobramycin added to known creatinine concentrations by the Jaffe reaction based creatinine estimation.

RESULTS

Only streptomycin above therapeutic concentrations of 10mg/mL interfered in the Jaffe reaction and acted as non-creatinine chromogen.

CONCLUSIONS

Therapeutic doses of the aminoglycosides do not form non-creatinine chromogens.