Kineothrix sedimenti sp. nov., a 3-hydroxybutyrate-producing bacterium isolated from sediment of the meromictic Lake Pavin.

An anaerobic, spore-forming, 3-hydroxybutyrate (3-HB)-producing bacterium, strain IPX_CKT, was isolated from sediment of a meromictic lake located in Massif Central (France). Cells were rods, forming filamentous chains which were observed moving under the microscope. Strain IPX_CKT utilized a wide variety of carbohydrates, but not raffinose, rhamnose and starch. Hydrogen (H2), 3-HB, acetate and ethanol were the main fermentative end-products from growth in medium containing glucose. Strain IPX_CKT grew optimally at 37 °C and pH 7. Its closest phylogenetic relative was Kineothrix alysoides (16S rRNA gene sequence identity 98.7%, isDDH 34.6%, ANIb 87.4%). The genomic DNA G+C content was 43.0 mol%. As for K. alysoides, whole-genome sequencing suggested that strain IPX_CKT is capable of fixing nitrogen (N2). However, strain IPX_CKT carried a five-nif-gene-set (nifHDKEB), not present in K. alysoides. Genome sequence also showed a high number of encoded chemotaxis receptors (42 genes, the second highest in the family Lachnospiraceae after K. alysoides). Based on phenotypic, genomic, phylogenetic and chemotaxonomic analyses, it is proposed that a novel species, Kineothrix sedimenti sp. nov., be created, with strain IPX_CKT (DSM 118044T, CIP 112511T) as the type strain.