Optimal dose of bone marrow mesenchymal stem cell transplantation for experimental ulcerative colitis.

OBJECTIVE

To investigate the optimal dose of bone marrow mesenchymal stem cell-transplantation for the ulcerative colitis rat.

METHODS

The BMSC of SD rat were isolated, cultured and labelled with DAPI. SD rats were randomly distributed into 3 groups, Colitis was induced with immune-combined TNBS/ethanol in group A、B、C, 3 groups received caudal vein injection of 1 mL fluids, which contain cell number 1 × 106、5 × 106、1 × 107 separately. 5 rats in each group were sacrificed at day 7 and 14 after injection, Cryostat sections of gut, The number of BMSCs in colon and normal tissue surrounded was observed with fluorescent microscope.

RESULTS

The DAPI marked BMSCs could been seen in the colic mucosa in each group on day 7、14, more cells in colon than the surrounding normal tissue, compared with 1 × 106 group, More cells in 5 × 106 group (P < 0.05), there were no significant difference (P > 0.05) between 5 × 106 group and 1 × 107 group. There were more cells in colon on 14 day than 7 day, and less in the surrounding normal tissue on 14 day than 7 day.

CONCLUSIONS

The density 5 × 106 is proper of bone mesenchymal stem cells for treatment of ulcerative colitis.