Genetic variability within the VP1 coding region of echovirus type 30 isolates.

Genetic relationships of the prototype Bastianni strain of 1958 and of 13 echovirus type 30 (ECV30) isolates associated with meningitis cases in Germany during a period from 1966 to 1997 were investigated using direct sequencing of amplicons derived from a part of the capsid protein VP1 gene. Sequences were aligned both with each other and with known sequences of other type 30 echovirus strains. Phylogenetic analysis indicated that isolates investigated in this work fell into at least three genetic clusters apart from the prototype Bastianni strain. This suggests that genetically distinct groups of ECV30 variants have developed over time.