[Development of a colloidal gold immunochromatographic test strip for detecting gB antibodies of porcine pseudorabies virus].

This study aims to establish a colloidal gold immunochromatographic test strip for the detection of glycoprotein B (gB) antibodies of porcine pseudorabies virus (PRV), thus providing a simple and rapid technique for the detection of PRV gB antibodies. The monoclonal antibodies to gB were prepared by the hybridoma technology. The gB protein was coupled with colloidal gold, and then gB and its monoclonal antibodies were separately sprayed onto a nitrocellulose membrane as the test line and control line. After optimization of conditions, a colloidal gold immunochromatographic test strip for detecting PRV gB antibodies was successfully developed. The specificity, sensitivity, stability, and concordance of the test strip were subsequently validated. The sensitivity of the test strip was 1:128. There was no cross-reactivity with positive sera of porcine reproductive and respiratory syndrome virus, classical swine fever virus, senecavirus A, and foot-and-mouth disease virus. The test strip could tolerate 37 °C for at least 72 d. In the test of 96 clinical pig serum samples, the concordance rate of the test strip results with the results of the commercial ELISA kit was 92.71%. The colloidal gold immunochromatographic test strip prepared in this study for PRV gB antibodies has good specificity, high sensitivity, and simple result interpretation, being suitable for rapid detection of porcine pseudorabies in the field.