Profile of PRMT-1 gene polymorphism in hemodialysis patients with increased ADMA levels.

AIM: to determine the distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis patients.

METHODS

genotyping of PRMT-1 polymorphism was performed in 57 hemodialysis patients at Al Irsjad Hospital. All participants were recruited for physical examination, questionnaire, and collection of 5 mL fasting venous blood. The blood was treated with phenol-chloroform extraction of genomic DNA. The candidate's single nucleotide polymorphisms (SNPs) were genotyped by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The ADMA plasma levels was determined by ELISA and all biochemical indicators of serum were examined.

RESULTS

fifty-seven hemodialysis patients participated in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism at sequence 5837 as there were different genotypes between C and G.

CONCLUSION

the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.