Exocytosis of secretory granules in the juxtaglomerular granular cells of kidneys.

BACKGROUND

There is little agreement as to the secretory process of renin granules in juxtaglomerular granular cells (JG cells) of kidneys, although a large number of studies of the regulation of renin secretion have been reported.

METHODS

The structural correlation between the stimuli and the secretory process was examined in mouse JG cells on renal cortical slices incubated with the beta-adrenergic agonist, isoproterenol; the loop diuretic, furocemide; the Ca2+ chelator, EGTA; and the actin filament-disrupting agent, cytochalasin B.

RESULTS AND

CONCLUSIONS

Treatment with isoproterenol (10(-5)-10(-3) M) or furocemide (10(-3) M) in Ca(2+)-containing medium did not significantly affect the ultrastructure of JG cells. In slices incubated with isoproterenol or furocemide in the Ca(2+)-free medium, JG cells occasionally contained a few electron-lucent granules at the cell periphery in addition to the electron-dense mature granules observed in the control slices. On rare occasions, the JG cells displayed omega-shaped cavities with electron-lucent matrices, a feature similar to the contents of electron-lucent granules. Cytochalasin B markedly promoted the effects of these stimulants in Ca(2+)-free medium. These findings suggest that participation of actin filament disassembly in the exocytotic process of the mature granules in JG cells.