Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2016
A novel fluorescent probe for rapid and sensitive detection of hydrogen sulfide in living cells.
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Pan Jian, Xu Junchao, Zhang Youlai, Wang Liang, Qin Caiqin, Zeng Lintao, Zhang Yue
Аннотация
A novel fluorescent probe for H2S was developed based on a far-red emitting indole-BODIPY, which was decorated with morpholine and 2,4-dinitrobenzenesulfonyl (DNBS) group. This probe showed rapid response (t1/2=3min), high selectivity and sensitivity for H2S with significant colorimetric and fluorescence OFF-ON signals, which was triggered by cleavage of 2,4-dinitrobenzenesulfonyl group. This probe could quantitatively detect the concentrations of H2S ranging from 0 to 60μM, and the detection of limit was found to be as low as 26nM. Cell imaging results indicated that the probe could detect and visualize H2S in the living cells.