[INFLUENCE OF HYDROGEN SULFIDE ON BLOOD OXYGEN PARAMETERS DURING HEPATIC ISCHEMIA-REPERFUSION IN RATS].

Tissue relative "hyperoxia" during reperfusion can cause oxidative stress due to oxygen participation in free radical processes. The influence of the hydrogen sulfide on the blood oxygen was investigated during hepatic ischemia(30 min, m.Pringle) and reperfusion(120 min) in rats. The parameters of blood oxygen (р50, Hb, MetHb, HbCO, pCO2,pO2, pH, TCO2, ABE, SBC), summary nitrite/nitrate level, plasma hydrogen sulfide and transaminases (ALT, AST) as a marker of liver damage were estimated. It's detected, that a single injection of sodium hydrosulfide (14 μmol/kg, 5 min before reperfusion) improves blood oxygen parameters (p50, pH, TCO2, ABE, SBC), plasma hydrogen sulfide and decreases transaminase levels during hepatic ischemia-reperfusion in rats. It was no changes in the MetHb or HbCO concentrations after sodium hydrosulfide infusion. The protective effect of hydrogen sulfide can be bind with increased hemoglobin oxygen affinity in the blood during reperfusion.