Reprogramming Patient-Derived urine cells into iPSCs for Anti-GAD65 autoimmune encephalitis research.

Urine cells from a patient with anti-GAD65autoantibody-associated autoimmune limbic encephalitis (ALE) were reprogrammed intoiPSCline IUFi020-A. Pluripotency was confirmed throughhiPSCore analysis while G-banding and CNVanalysis demonstrated that IUFi020-A exhibits characteristic features of a bona fide iPSC line with no genetic changes compared to the donor urine cells. STRanalysis further confirmed that IUFi020-A was derived from the parental urine cells. Additional characterization verified the absence of plasmidintegration and mycoplasma contamination.IUFi020-Aline provides a non-invasive alternative to fibroblast-based reprogramming and serves as a valuable model for investigating the pathogenic mechanisms of anti-GAD65-associated ALE.