In situ observation of ninhydrin and phenylhydrazine reaction in solution by FTIR.

The current work was performed for hydrazone formation reaction of ninhydrin with phenylhydrazine in chloroform by using "background defining method" in FTIR liquid cell. The method allowed following the consumption and formation of the reagent and product at the same time. Negative absorption bands are based on reagent consumption and positive absorbances are based on product formation. The method was applied for two different mol ratios (1:1, 1:3) of reagent. Both ratios resulted in same product (1,2,3-tris-phenylhydrazonoindan). The results showed that, phenylhydrazine react with all of the carbonyl groups in ninhydrin at both run.