Biological & pharmaceutical bulletin · 2013
Expression and secretion of cholera toxin B subunit in lactobacilli.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Okuno Takahiro, Kashige Nobuhiro, Satho Tomomitsu, Irie Keiichi, Hiramatsu Yukihiro, Sharmin Tanjina, Fukumitsu Yuki, Uyeda Saori, Yamada Seitaro, Harakuni Tetsuya, Miyata Takeshi, Arakawa Takeshi, Imoto Masumi, Toda Akihisa, Nakashima Yukihiko, Miake Fumio
Аннотация
Lactic acid bacteria (LAB) are used in various fields, including in food and medical supplies. There has been a great deal of research into vaccine development using LAB as carriers due to their "generally recognized as safe" status. Cholera is an infectious disease that causes diarrhea due to cholera toxin (CT) produced by Vibrio cholerae. The pentameric cholera toxin B (CTB) subunit has no toxicity, and is used as an antigen in cholera vaccines and as a delivery molecule in vaccines to various diseases. In this study, we generated recombinant LAB expressing and secreting CTB. Here, we first report that CTB expressed and secreted from LAB bound to GM1 ganglioside. The secreted CTB was purified, and its immunogenicity was determined by intranasal administration into mice. The results of the present study suggested that it may be useful as the basis of a new oral cholera vaccine combining LAB and CTB.