A rapid two-step protocol for isolation of DNA-free, high-integrity RNA from gram-negative bacteria.

A two-step protocol employing Tris-equilibrated phenol (pH 4.5) and sodium dodecyl sulfate was optimized to efficiently isolate DNA-free, intact RNA from gram-negative bacteria. This method reduced processing time and reagent use, thereby minimizing RNA degradation and contamination. The extracted RNA was suitable for gene expression profiling.