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Войти в исследования Tropical medicine and infectious disease · 2023
First Isolation and Genome Sequence Analysis of West Nile Virus in Mosquitoes in Brazil.
СтатьяНазвание и аннотация: Перевод в очереди
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Neto Joaquim Pinto Nunes, Reis Lúcia Aline Moura, Freitas Maria Nazaré Oliveira, do Nascimento Bruna Laís Sena, das Chagas Liliane Leal, da Costa Hernan Hermes Monteiro, Rodrigues Jéssica Cecília Pinheiro, Braga Camila Margalho, da Silva Eliana Vieira Pinto, Silva Sandro Patroca, Martins Lívia Caricio
Аннотация
West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.