Single-nucleus transcriptomic data of susceptible and resistant tobacco stems after black shank infection.

Black shank, caused by Phytophthora nicotianae, is a major disease in tobacco production. Previous transcriptomic work has used Nicotiana tabacum L. cv. Honghuadajinyuan (HD) as a susceptible tobacco material in black shank research. Presented here is a single-nucleus RNA sequencing (snRNA-seq) dataset generated from stem tissues of two tobacco accessions with contrasting responses to black shank, namely the susceptible cultivar HD and the black shank-resistant near-isogenic line HDR7 derived from HD. Stem tissues surrounding the inoculation site were collected at 48 h post inoculation with P. nicotianae race 0 and used for nucleus isolation, library construction, and sequencing. Because plant tissues are often difficult to dissociate into intact single cells, nucleus-based transcriptomic profiling provides a practical way to generate cell-resolved expression data from these samples. The dataset contains snRNA profiles from infected tobacco stems of both susceptible and resistant materials and can be reused for cell-type annotation, marker screening, comparative analysis of transcriptional features between accessions, and integration with future tobacco stem or disease-related single-cell and single-nucleus transcriptomic datasets.