Evaluation of Endolysin Activity Using Nephelometric Assays with Whole Bacterial Cells and Isolated Peptidoglycan.

This chapter presents a set of protocols for evaluating endolysin activity through nephelometric turbidity assays. Nephelometric assays measure the decrease in optical density over time using either heat-inactivated bacterial cells or purified peptidoglycan (PG) as substrates, offering a rapid and quantitative approach to assess lytic enzyme function. Complementary methods for PG extraction, purification, and dye-labeling are also included. Together, these protocols support consistent and reproducible assessment of endolysin activity across diverse experimental contexts.