Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026
[Isolation and identification of Pediococcus acidilactici BPA03 derived from human milk and evaluation of its antioxidant and anti-aging probiotic properties].
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Cao Longbin, Huang Peixiang, Zhang Zikang, Sun Weimin, Qiu Feng
Аннотация
OBJECTIVES: To identify Pediococcus acidilactici BPA03 strain isolated from healthy women's milk and evaluate its safety and probiotic properties.
METHODS: BPA03 strain was isolated from breast milk using dilution plating and streaking on MRS agar. Identification was performed using Gram staining, biochemical assays (BD card), 16S rRNA sequencing, and phylogenetic analysis. Safety assessment of the strain was conducted by evaluation of its hemolytic activity on blood agar, antibiotic susceptibility (Kirby-Bauer), and acute toxicity in zebrafish embryos. The probiotic properties of the bacterium were evaluated by measuring lactic acid production and assessing its protective effects against UV-induced photoaging and D-galactose-induced aging in zebrafish models.
RESULTS: The BPA03 strain was Gram-positive with a spherical or ovoid shape, and identified as Pediococcus acidilactici. This strain showed no hemolytic activity and was sensitive to all the tested antibiotics (penicillin, ampicillin, and vancomycin). Growth curve analysis showed a lag phase of 2 h, a logarithmic phase starting from 4 to 12 h, and a stationary phase after 20 h in aerobic culture. Lactic acid concentration reached 23.6 mmol/L after culture for 8 h in MRS medium. In zebrafish embryos, BPA03 exhibited no significant acute toxicity, and the embryos showed a hatching rate above 95% even in the presence of 10⁸ CFU/mL BPA03 (LD₅₀>10⁸ CFU/mL). In UV-exposed zebrafish models, BPA03 obviously alleviated UV-induced caudal fin damage, significantly reduced reactive oxygen species and malondialdehyde levels, enhanced antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase), and downregulated mRNA expression of pro-inflammatory cytokines (IL‑1β, IL‑6, IL‑8, and TNF‑α).
CONCLUSIONS: Pediococcus acidilactici BPA03 strain isolated from breast milk of healthy women exhibits good probiotic properties and safety.
METHODS: BPA03 strain was isolated from breast milk using dilution plating and streaking on MRS agar. Identification was performed using Gram staining, biochemical assays (BD card), 16S rRNA sequencing, and phylogenetic analysis. Safety assessment of the strain was conducted by evaluation of its hemolytic activity on blood agar, antibiotic susceptibility (Kirby-Bauer), and acute toxicity in zebrafish embryos. The probiotic properties of the bacterium were evaluated by measuring lactic acid production and assessing its protective effects against UV-induced photoaging and D-galactose-induced aging in zebrafish models.
RESULTS: The BPA03 strain was Gram-positive with a spherical or ovoid shape, and identified as Pediococcus acidilactici. This strain showed no hemolytic activity and was sensitive to all the tested antibiotics (penicillin, ampicillin, and vancomycin). Growth curve analysis showed a lag phase of 2 h, a logarithmic phase starting from 4 to 12 h, and a stationary phase after 20 h in aerobic culture. Lactic acid concentration reached 23.6 mmol/L after culture for 8 h in MRS medium. In zebrafish embryos, BPA03 exhibited no significant acute toxicity, and the embryos showed a hatching rate above 95% even in the presence of 10⁸ CFU/mL BPA03 (LD₅₀>10⁸ CFU/mL). In UV-exposed zebrafish models, BPA03 obviously alleviated UV-induced caudal fin damage, significantly reduced reactive oxygen species and malondialdehyde levels, enhanced antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase), and downregulated mRNA expression of pro-inflammatory cytokines (IL‑1β, IL‑6, IL‑8, and TNF‑α).
CONCLUSIONS: Pediococcus acidilactici BPA03 strain isolated from breast milk of healthy women exhibits good probiotic properties and safety.