Evaluation of a Luminex-Based Multiplex Immunoassay of Hemorrhagic Fever Viruses in Senegal.

Given the growing threat posed by viral hemorrhagic fevers, the development of surveillance tools is crucial to provide accurate and rapid solutions. Public health response involves risk assessment as well as effective and sustainable surveillance to ensure downstream communication and preparedness. A serological approach that offers high precision and throughput, cost efficiency, and multiplexing capacity is critical. In this work, we evaluated a Luminex-based multiplex microsphere immunoassay for five hemorrhagic fever viruses (HFVs) among the World Health Organization (WHO) blueprint. This five-plex MagPix immunoassay confirmed the presence of Rift Valley fever and Crimean-Congo hemorrhagic fever, but also revealed the exposure of human populations to hantaviruses in Senegal, underscoring the importance of regular serosurveillance in the identification of HFV hotspots.