The Journal of prosthetic dentistry · 2026
Influence of protocol and number of sterilization cycles on the accuracy of an additively manufactured extraoral scan body fabricated with two biocompatible materials.
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Revilla-León Marta, Oh Kyung Chul, Barmak Abdul B, Park Ji-Man, Kois John C
Аннотация
STATEMENT OF PROBLEM: Biocompatible printed extraoral scan bodies can be used to integrate a 3-dimensional virtual patient and to transfer the maxillary cast to the virtual articulator. However, the influence of sterilization procedures on the dimensional stability of these printed devices remains unknown.
PURPOSE: The purpose of this in vitro study was to assess the influence of 2 sterilization protocols (short and long) and number of cycles (1 to 10) on the accuracy of a printed extraoral scan body fabricated from 2 biocompatible printing materials.
MATERIAL AND METHODS: A virtual design of an extraoral scan body was obtained. Two groups were defined by printing material: biocompatible white (BioMed White) (BMW group) and custom tray (DentaTRAY) (DT group) materials. Two subgroups were created depending on the sterilization protocol: short (3 minutes at 134 °C) and long (15 minutes at 121 °C) (n=10). Specimens in the BMW group were printed with a Form 4B, and those in the DT group with a Pro 4K) by following the printing strategy recommended by each manufacturer. Each specimen was numbered and scanned by using a laboratory scanner (control). These procedures were repeated until 10 autoclave cycles had been completed. Volumetric discrepancies among consecutive sterilization cycles for each specimen were calculated by using the root mean square (RMS) error with the cycle 0 scan as a reference. Three-way ANOVA for repetitive measures and Tukey tests were used to analyze trueness. The Levene test was used to analyze precision (α=.05).
RESULTS: Trueness discrepancies were found among the sterilization cycles (P<.001), groups (P<.001), and subgroups (P<.001) with a significant group×subgroup interaction (P<.001). The BMW group obtained better trueness than the DT group. The short-cycle subgroup obtained better trueness than the long-cycle group. Each cycle obtained a statistically significantly worse trueness than the previous sterilization cycle. Precision discrepancies were found among the groups and subgroups tested (P<.05). In the sterilization cycle 3, the BMW group obtained worse precision than the DT group (P=.005), but, in the sterilization cycles 6, 7, 8, and 9, the DT group had significantly worse precision than the BMW group (P<.001). In the sterilization cycle 5, 6, 7, 8, and 9, the short-cycle subgroup had worse precision than the long-cycle subgroup.
CONCLUSIONS: The sterilization protocol and autoclave cycles affected the trueness and precision of the extraoral scan body, regardless of the biocompatible resin selected. As the number of autoclave cycles increased, the trueness and precision of the extraoral printed scan body and volumetric stability decreased.
PURPOSE: The purpose of this in vitro study was to assess the influence of 2 sterilization protocols (short and long) and number of cycles (1 to 10) on the accuracy of a printed extraoral scan body fabricated from 2 biocompatible printing materials.
MATERIAL AND METHODS: A virtual design of an extraoral scan body was obtained. Two groups were defined by printing material: biocompatible white (BioMed White) (BMW group) and custom tray (DentaTRAY) (DT group) materials. Two subgroups were created depending on the sterilization protocol: short (3 minutes at 134 °C) and long (15 minutes at 121 °C) (n=10). Specimens in the BMW group were printed with a Form 4B, and those in the DT group with a Pro 4K) by following the printing strategy recommended by each manufacturer. Each specimen was numbered and scanned by using a laboratory scanner (control). These procedures were repeated until 10 autoclave cycles had been completed. Volumetric discrepancies among consecutive sterilization cycles for each specimen were calculated by using the root mean square (RMS) error with the cycle 0 scan as a reference. Three-way ANOVA for repetitive measures and Tukey tests were used to analyze trueness. The Levene test was used to analyze precision (α=.05).
RESULTS: Trueness discrepancies were found among the sterilization cycles (P<.001), groups (P<.001), and subgroups (P<.001) with a significant group×subgroup interaction (P<.001). The BMW group obtained better trueness than the DT group. The short-cycle subgroup obtained better trueness than the long-cycle group. Each cycle obtained a statistically significantly worse trueness than the previous sterilization cycle. Precision discrepancies were found among the groups and subgroups tested (P<.05). In the sterilization cycle 3, the BMW group obtained worse precision than the DT group (P=.005), but, in the sterilization cycles 6, 7, 8, and 9, the DT group had significantly worse precision than the BMW group (P<.001). In the sterilization cycle 5, 6, 7, 8, and 9, the short-cycle subgroup had worse precision than the long-cycle subgroup.
CONCLUSIONS: The sterilization protocol and autoclave cycles affected the trueness and precision of the extraoral scan body, regardless of the biocompatible resin selected. As the number of autoclave cycles increased, the trueness and precision of the extraoral printed scan body and volumetric stability decreased.