Photodiagnosis and photodynamic therapy · 2026
Methylene blue-enhanced photodynamic and sonodynamic therapy againstStaphylococcus aureus: From laboratory research to clinical evaluation.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Kolarova Hana, Valkova Lucie, Kolarikova Marketa, Urban Jan, Vecerova Renata, Bajgar Robert, Langova Katerina, Hosek Jiri, Tomankova Katerina Barton, Dilenko Hanna, Kolar Milan, Jirova Dagmar
Аннотация
BACKGROUND: Antibiotic resistance among multidrug-resistant pathogens necessitates alternative antimicrobial strategies. Photodynamic therapy (PDT) and sonodynamic therapy (SDT), particularly with methylene blue (MB), show promise but remain underexplored in clinical contexts.
METHODS: We conducted an in vitro evaluation of MB-PDT and SDT against Staphylococcus aureus, a major cause of skin and soft tissue infections. Assays included growth curve analysis, scanning electron microscopy (SEM) to assess bacterial ultrastructure. A pilot clinical study in 20 healthy volunteers assessed in vivo antimicrobial efficacy and tolerability under standardized conditions.
RESULTS: In vitro, MB demonstrated antimicrobial activity under PDT, with further potentiation by SDT and repeated irradiation. SEM confirmed structural disruption and lysis of S. aureus following combination therapy. In vivo, both MB-PDT and PDT+SDT achieved significant bacterial log reductions compared with baseline (p < 0.0001), with the highest efficacy observed under repeated irradiation plus SDT. The most significant effect was observed with repeated irradiation (2 × 30 J.cm⁻²) in combination with ultrasound (1 MHz, 1 W/cm²), with an average reduction of 2.424 log₁₀ (SD = 0.652).
CONCLUSIONS: MB-mediated PDT and PDT+SDT are safe, well tolerated, and effective against S. aureus on skin. These approaches provide localized antimicrobial activity independent of antibiotic resistance and warrant further optimization for clinical application.
METHODS: We conducted an in vitro evaluation of MB-PDT and SDT against Staphylococcus aureus, a major cause of skin and soft tissue infections. Assays included growth curve analysis, scanning electron microscopy (SEM) to assess bacterial ultrastructure. A pilot clinical study in 20 healthy volunteers assessed in vivo antimicrobial efficacy and tolerability under standardized conditions.
RESULTS: In vitro, MB demonstrated antimicrobial activity under PDT, with further potentiation by SDT and repeated irradiation. SEM confirmed structural disruption and lysis of S. aureus following combination therapy. In vivo, both MB-PDT and PDT+SDT achieved significant bacterial log reductions compared with baseline (p < 0.0001), with the highest efficacy observed under repeated irradiation plus SDT. The most significant effect was observed with repeated irradiation (2 × 30 J.cm⁻²) in combination with ultrasound (1 MHz, 1 W/cm²), with an average reduction of 2.424 log₁₀ (SD = 0.652).
CONCLUSIONS: MB-mediated PDT and PDT+SDT are safe, well tolerated, and effective against S. aureus on skin. These approaches provide localized antimicrobial activity independent of antibiotic resistance and warrant further optimization for clinical application.