Journal of oral science · 2026
Development of fish scale collagen-immobilized poly(lactic acid) films using chemical treatment with hexamethylenediamine.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Fuse Megumi
Аннотация
PURPOSE: This study aimed to evaluate how immobilizing tilapia fish scale type 1 collagen onto poly(lactic acid) (PLA), following chemical modification with hexamethylenediamine (HMDA), affects the adhesion and proliferation of mouse osteoblast-like cells (OLCs).
METHODS: PLA films were chemically modified by HMDA immersion to introduce amino groups (-NH2) (PLA-NH2). Tilapia fish scale type 1 collagen was subsequently immobilized onto the PLA-NH2 surface via a water-soluble carbodiimide condensation reaction, producing (TFColl-PLA-NH2). Surface properties of the films were characterized using contact angle measurements and X-ray photoelectron spectroscopy (XPS). Biological evaluation was conducted by culturing OLCs on PLA, PLA-NH2, and TFColl-PLA-NH2 films, followed by quantification of cell numbers.
RESULTS: The contact angles of PLA-NH2 and TFColl-PLA-NH2 were significantly lower than PLA (P < 0.05). XPS confirmed successful immobilization of tilapia collagen onto the PLA surface. At all time points, TFColl-PLA-NH2 showed the highest cell numbers, followed by PLA-NH2, while PLA had the lowest (P < 0.05). PLA-NH2 promoted calcification more effectively than PLA (P < 0.05), and TFColl- PLA-NH2 further enhanced calcification compared with PLA-NH2 (P < 0.05).
CONCLUSION: PLA surface treatment with HMDA is an effective and straightforward method. Tilapia fish scale type 1 collagen has potential for application as a novel biomaterial source.
METHODS: PLA films were chemically modified by HMDA immersion to introduce amino groups (-NH2) (PLA-NH2). Tilapia fish scale type 1 collagen was subsequently immobilized onto the PLA-NH2 surface via a water-soluble carbodiimide condensation reaction, producing (TFColl-PLA-NH2). Surface properties of the films were characterized using contact angle measurements and X-ray photoelectron spectroscopy (XPS). Biological evaluation was conducted by culturing OLCs on PLA, PLA-NH2, and TFColl-PLA-NH2 films, followed by quantification of cell numbers.
RESULTS: The contact angles of PLA-NH2 and TFColl-PLA-NH2 were significantly lower than PLA (P < 0.05). XPS confirmed successful immobilization of tilapia collagen onto the PLA surface. At all time points, TFColl-PLA-NH2 showed the highest cell numbers, followed by PLA-NH2, while PLA had the lowest (P < 0.05). PLA-NH2 promoted calcification more effectively than PLA (P < 0.05), and TFColl- PLA-NH2 further enhanced calcification compared with PLA-NH2 (P < 0.05).
CONCLUSION: PLA surface treatment with HMDA is an effective and straightforward method. Tilapia fish scale type 1 collagen has potential for application as a novel biomaterial source.