Journal of conservative dentistry and endodontics · 2026
Correlation of dentinal penetration and microleakage of bioceramic with calcium hydroxide and resin based root canal sealers- A CLSM study.
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Muktawat Kirtika, Kumari Manju, Kochhar Rohit
Аннотация
CONTEXT: The role of Root canal sealers is a very crucial role in endodontic therapy by forming a hermetic seal within the canal, filling minor voids between the dentinal wall and gutta-percha to prevent microleakage from bacterial infiltration from the oral cavity and tissues of periapical areas.
AIM: This study aims to compare and evaluate the depth of penetration and microleakage of AH Plus, Bio-C, Sealapex, and MTA Fillapex using a confocal laser scanning microscope (CLSM).
SUBJECTS AND METHODS: This study evaluated sealer penetration and microleakage in 170 single-rooted teeth divided into two groups (n = 85 each). Group A assessed sealer penetration of Group I: MTA Fillapex, Group II: Selapex, Group III: Bio-C, Group IV: AH Plus, and Group V: Control group (obturated without sealer) using 0.1% Rhodamine B-labeled sealers, and CLSM was done at 3 mm and 5 mm from the apex. Group B measured microleakage in the above-mentioned 5 groups, after 7-day immersion of samples in 0.1% Rhodamine B and evaluating dye penetration longitudinally from the cemento-dentinal junction.
STATISTICAL ANALYSIS: Data were analyzed using paired t-tests, Tukey Honestly Significant Difference, ANOVA, and Pearson's correlation as statistical tools.
RESULTS: Group III has demonstrated the highest sealer penetration at both 3 mm and 5 mm levels, and exhibits the best microleakage resistance. However, minimum penetration has been shown by Group I and Group II at 3 mm and 5 mm, respectively. Group II has shown the highest microleakage among the sealers.
CONCLUSION: Bio-C sealer performed best, whereas Sealapex has shown the worst both in sealer penetration and microleakage.
AIM: This study aims to compare and evaluate the depth of penetration and microleakage of AH Plus, Bio-C, Sealapex, and MTA Fillapex using a confocal laser scanning microscope (CLSM).
SUBJECTS AND METHODS: This study evaluated sealer penetration and microleakage in 170 single-rooted teeth divided into two groups (n = 85 each). Group A assessed sealer penetration of Group I: MTA Fillapex, Group II: Selapex, Group III: Bio-C, Group IV: AH Plus, and Group V: Control group (obturated without sealer) using 0.1% Rhodamine B-labeled sealers, and CLSM was done at 3 mm and 5 mm from the apex. Group B measured microleakage in the above-mentioned 5 groups, after 7-day immersion of samples in 0.1% Rhodamine B and evaluating dye penetration longitudinally from the cemento-dentinal junction.
STATISTICAL ANALYSIS: Data were analyzed using paired t-tests, Tukey Honestly Significant Difference, ANOVA, and Pearson's correlation as statistical tools.
RESULTS: Group III has demonstrated the highest sealer penetration at both 3 mm and 5 mm levels, and exhibits the best microleakage resistance. However, minimum penetration has been shown by Group I and Group II at 3 mm and 5 mm, respectively. Group II has shown the highest microleakage among the sealers.
CONCLUSION: Bio-C sealer performed best, whereas Sealapex has shown the worst both in sealer penetration and microleakage.