[Clinical practice guidelines for the in vitro preservation and repair of severed limbs (2025 edition)].

Replantation of amputated limbs is the most effective treatment for severe limb injuries, and allogeneic limb transplantation is also one of the potentially most effective methods to address limb amputation injuries in the future. The preservation and repair of severed limbs outside the body is a key factor in improving the success rate of limb replantation and transplantation. Currently, the techniques for preserving and repairing severed limbs outside the body include static refrigeration preservation technology, immersion in cryoprotective solution technology, deep cryogenic freezing technology, mechanical perfusion technology, temporary vascular shunt technology, autologous ectopic grafting technology, and experimental-stage xenogeneic cross-circulation technology. By optimizing key parameters such as temperature, pressure, and preservation solution, these techniques can extend the preservation time of the limbs and increase the success rate of replantation. Traditional techniques have a short preservation time and cannot meet clinical needs. Emerging technologies are in the ascendant and are subject to different opinions. National Clinical Research Center for Orthopedics Sports Medicine & Rehabilitation, the Microsurgery Branch of Chinese Medical Association and the Microsurgery Branch of Beijing Orthopedic Association, based on current evidence-based medical evidence, have formulated the "Clinical practice guidelines for the in vitro preservation and repair of severed limbs (2025 edition)". This guideline systematically evaluates a series of literature and related evidence-based medical research evidence published domestically and internationally in the field of organ preservation in recent years, and adds evidence-based medical research data based on the Chinese population, with the aim of further standardizing the relevant techniques for ex vivo preservation of amputated limbs in China, for industry reference.