Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012
Emergence of a unique recombinant form of HIV-1 from Manipur (India).
Клинический случайСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Sarkar Roni, Sarkar Kamalesh, Brajachand Singh N, Manihar Singh Y, Mitra Debashis, Chakrabarti Sekhar
Аннотация
BACKGROUND: The AIDS epidemic in Manipur, India, manifests unique features, having co-circulation of B and C HIV-1 subtypes along with recombinant forms. Manipur has the highest incidence of HIV-1 infection compared to the other states of India, but limited information is available regarding the full-length sequence of HIV-1 recombinants.
OBJECTIVES: To characterize the near full-length genome of a novel recombinant HIV-1 strain from an injecting drug user of Manipur.
STUDY DESIGN: Viral RNA, extracted from the plasma of a male injecting drug user aged 35, was diagnosed with HIV-1 infection. Near full-length genome was amplified by polymerase chain reaction using primer walking approach. Phylogenetic relationships were determined with neighbor-joining trees. The recombination break points were detected using boot scan and Simplot analyses.
RESULTS: This recombinant predominantly had subtype C genome and exhibited mosaic structures with subtype B insertions at three different positions of HIV-1 genome. Simplot analysis of near full-length genome sequence from the recombinant HIV-1 strain, MAN86 exhibited similarity with the sequence of C.IN.93.93IN905 in its subtype C backbone, while the subtype B insertions showed resemblance with the sequence of B.TH.99.99(TH)_C1416.
CONCLUSIONS: This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from India and Thailand.
OBJECTIVES: To characterize the near full-length genome of a novel recombinant HIV-1 strain from an injecting drug user of Manipur.
STUDY DESIGN: Viral RNA, extracted from the plasma of a male injecting drug user aged 35, was diagnosed with HIV-1 infection. Near full-length genome was amplified by polymerase chain reaction using primer walking approach. Phylogenetic relationships were determined with neighbor-joining trees. The recombination break points were detected using boot scan and Simplot analyses.
RESULTS: This recombinant predominantly had subtype C genome and exhibited mosaic structures with subtype B insertions at three different positions of HIV-1 genome. Simplot analysis of near full-length genome sequence from the recombinant HIV-1 strain, MAN86 exhibited similarity with the sequence of C.IN.93.93IN905 in its subtype C backbone, while the subtype B insertions showed resemblance with the sequence of B.TH.99.99(TH)_C1416.
CONCLUSIONS: This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from India and Thailand.