Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2019
[Sterilization Effect of an Atmospheric Low Temperature Plasma Jet on Candida albicans Biofilm].
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Pu Qi-Kang, Liu Si-Jing, Huang Huan, Xiong Jing-Fei, Zhnang Li, Fang Zhi, Wang Chuan
Аннотация
OBJECTIVE: To evaluate the sterilization effect of new designed atmospheric low temperature plasma jet on Candida albicans ( C. albicans) biofilm.
METHODS: C. albicans was grown into the logarithmic phase, and then was added to polystyrene 24-well microtitre plate. The amount of germs were calculated by viable plate counting to determine the reproducibility of each biofilm well. The germs in biofilm were treated by plasma for different exposure time and then the survived germs were quantified by plate counting, the dead cells were determined by staining the biofilm with propidium iodide (PI), and the ultrastructural changes of the germs in biofilm were observed by transmission electron microscopy (TEM).
RESULTS: When incubated for 72 h, germs tightly polymerized and classical mature biofilm were formed. This atmospheric low temperature plasma jet could inactivate C. albicans biofilm within a short exposure time. C. albicans were 90% inactivated when treated 20 s and 55 s of plasma treatment reduced bacteria populations to undetectable levels. With the increase of treatment time, enlarged fluorescent positive area appeared, and more bacteria died with the extending of exposure. The TEM scanning results showed that the new plasma jet inactivated C. albicans biofilm mainly via disrupting cell envelopes and then leading the release of cellular components, thus resulting in loss of cell viability.
CONCLUSION: Plasma generated from atmospheric low temperature plasma jet could damage the cell structure of C. albicans and efficiently sterilize C. albicans biofilm.
METHODS: C. albicans was grown into the logarithmic phase, and then was added to polystyrene 24-well microtitre plate. The amount of germs were calculated by viable plate counting to determine the reproducibility of each biofilm well. The germs in biofilm were treated by plasma for different exposure time and then the survived germs were quantified by plate counting, the dead cells were determined by staining the biofilm with propidium iodide (PI), and the ultrastructural changes of the germs in biofilm were observed by transmission electron microscopy (TEM).
RESULTS: When incubated for 72 h, germs tightly polymerized and classical mature biofilm were formed. This atmospheric low temperature plasma jet could inactivate C. albicans biofilm within a short exposure time. C. albicans were 90% inactivated when treated 20 s and 55 s of plasma treatment reduced bacteria populations to undetectable levels. With the increase of treatment time, enlarged fluorescent positive area appeared, and more bacteria died with the extending of exposure. The TEM scanning results showed that the new plasma jet inactivated C. albicans biofilm mainly via disrupting cell envelopes and then leading the release of cellular components, thus resulting in loss of cell viability.
CONCLUSION: Plasma generated from atmospheric low temperature plasma jet could damage the cell structure of C. albicans and efficiently sterilize C. albicans biofilm.