Photodiagnosis and photodynamic therapy · 2019
Comparison study of root canal disinfection by cold plasma jet and photodynamic therapy.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Armand Ameneh, Khani Mohammadreza, Asnaashari Mohammad, AliAhmadi Atousa, Shokri Babak
Аннотация
INTRODUCTION: This study evaluated and compared the antibacterial effect of plasma and photodynamic therapy (PDT) in root canals infected with Enterococcus faecalis.
METHODS: One hundred single-rooted extracted human teeth (no treatment) were selected and sterilized. Root canals of teeth were prepared with the crown-down technique and contaminated with Enterococcus faecalis after sterilization, and incubated for one week. To investigate the effect of disinfection, 60 specimens were randomly selected for three disinfection groups: He and He/O2 plasma (n = 20) in 5-s groups (control, 2, 4, 6 and 8 min treatments) and PDT (n = 20) in 2-s groups (control and PDT treatment). Next, for comparison, 40 remaining teeth were divided into four groups: control, 8 min He plasma, 8 min He/O2, and PDT. Results of antibacterial action were reported by CFU/mL analysis and the structural changes of biofilm were evaluated by SEM.
RESULTS: All groups showed a significant reduction of bacteria after treatment, and He/O2 plasma was more effective against E. faecalis, followed by PDT and He plasma respectively, further confirmed by SEM.
CONCLUSION: He/O2 plasma is a strong sterilization method against E. faecalis biofilm and can, therefore, be an effective therapy in endodontics.
METHODS: One hundred single-rooted extracted human teeth (no treatment) were selected and sterilized. Root canals of teeth were prepared with the crown-down technique and contaminated with Enterococcus faecalis after sterilization, and incubated for one week. To investigate the effect of disinfection, 60 specimens were randomly selected for three disinfection groups: He and He/O2 plasma (n = 20) in 5-s groups (control, 2, 4, 6 and 8 min treatments) and PDT (n = 20) in 2-s groups (control and PDT treatment). Next, for comparison, 40 remaining teeth were divided into four groups: control, 8 min He plasma, 8 min He/O2, and PDT. Results of antibacterial action were reported by CFU/mL analysis and the structural changes of biofilm were evaluated by SEM.
RESULTS: All groups showed a significant reduction of bacteria after treatment, and He/O2 plasma was more effective against E. faecalis, followed by PDT and He plasma respectively, further confirmed by SEM.
CONCLUSION: He/O2 plasma is a strong sterilization method against E. faecalis biofilm and can, therefore, be an effective therapy in endodontics.