Photodiagnosis and photodynamic therapy · 2016
Streptococcus mutans photoinactivation using a combination of a high potency photopolymerizer and rose bengal.
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Leite Hadda Lyzandra Austríaco, Cavalcante Salma Ivanna Araújo, de Sousa Eduardo Martins, Gonçalves Letícia Machado, Paschoal Marco Aurélio
Аннотация
BACKGROUND: Long treatment periods to reach a substantial inactivation of microorganisms are one of the critical challenges in the photodynamic therapy field.
METHODS: Planktonic suspensions of Streptococcus mutans were treated in different groups: presence of rose bengal (RB at 2μM) and light exposure by a new high potency photopolymerizer (L at wavelength=440-480nm; dosimetry=96J/cm(2) - 40s of irradiation; potency density=1200mW/cm(2); dosage=48J) - RB+L+ (PDT), just with dye - RB+L-, just with light - RB-L+ and absence of light and dye RB-L- (control group). Aliquots of each group were transferred to Petri dishes to colony counting (CFU/mL) with the data transformed in log10. The results were submitted to ANOVA and Tukey test at 5%.
RESULTS: PDT group presented total eradication of microorganisms showing statistical difference with all the other groups (5.82log reduction≅99%).
CONCLUSION: The high potency photopolymerizer in pulsed emission at an extra short irradiation and low concentration of rose bengal could be considered as a progressive alternative to the control of S. mutans suspensions.
METHODS: Planktonic suspensions of Streptococcus mutans were treated in different groups: presence of rose bengal (RB at 2μM) and light exposure by a new high potency photopolymerizer (L at wavelength=440-480nm; dosimetry=96J/cm(2) - 40s of irradiation; potency density=1200mW/cm(2); dosage=48J) - RB+L+ (PDT), just with dye - RB+L-, just with light - RB-L+ and absence of light and dye RB-L- (control group). Aliquots of each group were transferred to Petri dishes to colony counting (CFU/mL) with the data transformed in log10. The results were submitted to ANOVA and Tukey test at 5%.
RESULTS: PDT group presented total eradication of microorganisms showing statistical difference with all the other groups (5.82log reduction≅99%).
CONCLUSION: The high potency photopolymerizer in pulsed emission at an extra short irradiation and low concentration of rose bengal could be considered as a progressive alternative to the control of S. mutans suspensions.