Proceedings of the National Academy of Sciences of the United States of America · 2019
CD4 receptor diversity in chimpanzees protects against SIV infection.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bibollet-Ruche Frederic, Russell Ronnie M, Liu Weimin, Stewart-Jones Guillaume B E, Sherrill-Mix Scott, Li Yingying, Learn Gerald H, Smith Andrew G, Gondim Marcos V P, Plenderleith Lindsey J, Decker Julie M, Easlick Juliet L, Wetzel Katherine S, Collman Ronald G, Ding Shilei, Finzi Andrés, Ayouba Ahidjo, Peeters Martine, Leendertz Fabian H, van Schijndel Joost, Goedmakers Annemarie, Ton Els, Boesch Christophe, Kuehl Hjalmar, Arandjelovic Mimi, Dieguez Paula, Murai Mizuki, Colin Christelle, Koops Kathelijne, Speede Sheri, Gonder Mary K, Muller Martin N, Sanz Crickette M, Morgan David B, Atencia Rebecca, Cox Debby, Piel Alex K, Stewart Fiona A, Ndjango Jean-Bosco N, Mjungu Deus, Lonsdorf Elizabeth V, Pusey Anne E, Kwong Peter D, Sharp Paul M, Shaw George M, Hahn Beatrice H
Аннотация
Human and simian immunodeficiency viruses (HIV/SIVs) use CD4 as the primary receptor to enter target cells. Here, we show that the chimpanzee CD4 is highly polymorphic, with nine coding variants present in wild populations, and that this diversity interferes with SIV envelope (Env)-CD4 interactions. Testing the replication fitness of SIVcpz strains in CD4+ T cells from captive chimpanzees, we found that certain viruses were unable to infect cells from certain hosts. These differences were recapitulated in CD4 transfection assays, which revealed a strong association between CD4 genotypes and SIVcpz infection phenotypes. The most striking differences were observed for three substitutions (Q25R, Q40R, and P68T), with P68T generating a second N-linked glycosylation site (N66) in addition to an invariant N32 encoded by all chimpanzee CD4 alleles. In silico modeling and site-directed mutagenesis identified charged residues at the CD4-Env interface and clashes between CD4- and Env-encoded glycans as mechanisms of inhibition. CD4 polymorphisms also reduced Env-mediated cell entry of monkey SIVs, which was dependent on at least one D1 domain glycan. CD4 allele frequencies varied among wild chimpanzees, with high diversity in all but the western subspecies, which appeared to have undergone a selective sweep. One allele was associated with lower SIVcpz prevalence rates in the wild. These results indicate that substitutions in the D1 domain of the chimpanzee CD4 can prevent SIV cell entry. Although some SIVcpz strains have adapted to utilize these variants, CD4 diversity is maintained, protecting chimpanzees against infection with SIVcpz and other SIVs to which they are exposed.