Frozen semen quality of Pasundan bulls with different individual variations.

BACKGROUND

Pasundan bulls are one of Indonesia's indigenous cattle breeds. The quality of frozen semen is essential for the success of artificial insemination (AI), as individual variations in parameters such as sperm motility, viability, and membrane integrity greatly influence reproductive outcomes.

AIM: The objective of this study was to evaluate the quality of frozen semen from Pasundan bulls with different individual variations.

METHODS

Frozen semen from eight Pasundan bulls was obtained from the West Java Regional Artificial Insemination Center. Sperm motility was assessed using computer-assisted sperm analysis (CASA; SpermVision®, Germany). Sperm viability and abnormalities were determined by eosin-nigrosine staining. Plasma membrane integrity was evaluated using the hypoosmotic swelling test, acrosome integrity was assessed using fluorescein isothiocyanate-propidium iodide, and protamine deficiency was measured using chromomycin A3 fluorescence staining.

RESULTS

Significant individual variations (p < 0.05) were found across all parameters of frozen semen quality, except for sperm abnormalities, which showed no significant differences (p > 0.05).

CONCLUSION

The results showed that individual variations significantly influence all quality parameters of frozen semen from Pasundan bulls, except for sperm abnormalities. Therefore, a thorough assessment of each bull is crucial to ensure the production of quality frozen semen.