Cryo letters · 2019
In Vitro and In Vivo Efficiency of Extenders Added to Thawed Stallion Semen.
СтатьяНазвание и аннотация: Перевод в очереди
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Sales F Abm, Ferreira-Silva J C, Vieira J I, Chaves M S, Caldas E L, Filho J Ps, Freitas V Jf, Oliveira M Al
Аннотация
BACKGROUND: Addition of extenders to thawed semen could improve fertility.
OBJECTIVE: To determine the efficiency of extenders to increase viability of thawed semen, measured by sperm parameters in vitro and pregnancy rates after artificial insemination (AI).
MATERIALS AND METHODS: Sperm motility and acrosin activity were measured during a thermoresistance test (TRT).
RESULTS: Progressive motility decreased (P<0.05) after 30 min in thawing semen treated with saline solution (SS) and only after 60 min with Tyrode's solution (TS) or freezing diluent (FD). The total motility decreased (P<0.05) after 60 min in thawed semen treated with SS, and after 90 min in thawed semen containing TS or FD. The acrosin activity decreased (P<0.05) after 60 min during the TRT, but there was no difference among treatments throughout the TRT. The pregnancy rates were similar among thawed-semen supplemented with SS, TS or FD.
CONCLUSION: The extenders neither improve sperm parameters nor enhance AI results.
OBJECTIVE: To determine the efficiency of extenders to increase viability of thawed semen, measured by sperm parameters in vitro and pregnancy rates after artificial insemination (AI).
MATERIALS AND METHODS: Sperm motility and acrosin activity were measured during a thermoresistance test (TRT).
RESULTS: Progressive motility decreased (P<0.05) after 30 min in thawing semen treated with saline solution (SS) and only after 60 min with Tyrode's solution (TS) or freezing diluent (FD). The total motility decreased (P<0.05) after 60 min in thawed semen treated with SS, and after 90 min in thawed semen containing TS or FD. The acrosin activity decreased (P<0.05) after 60 min during the TRT, but there was no difference among treatments throughout the TRT. The pregnancy rates were similar among thawed-semen supplemented with SS, TS or FD.
CONCLUSION: The extenders neither improve sperm parameters nor enhance AI results.