Cryo letters · 2018
Freezing of Stallion Semen: in vitro and in vivo Evaluation of Sperm Motility and Acrosin Activity in Frozen-thawed Semen with Addition of Post-diluent Extenders.
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Sales F, Ferreira-Silva J, Vieira J, Basto S, Filho P, Rocha J, Moura M T, Oliveira M
Аннотация
BACKGROUND: Post-diluents could potentially increase semen cryotolerance, but remain poorly explored in horses.
OBJECTIVE: The aim was to evaluate the efficiency of post-diluents on frozen-thawed semen viability of two stallions (S1-S2).
MATERIALS AND METHODS: The cryopreserved semen was thawed at 50°C for 40 seconds. Semen motility and acrosin activity (AA) were determined during the thermo-resistance test (TRT).
RESULTS: Progressive motility of S2 semen decreased after 60 and 90 minutes of TRT (TRT60 and TRT90) on the control compared to both post-diluents. The total motility of both S1 and S2 decreased on TRT60 and TRT90 semen control versus both Ringer and Merk post-diluents. The AA on S1 was higher than S2 throughout the TRT. Pregnancy rates after artificial insemination (AI) were similar among post-diluents and stallions.
CONCLUSION: Post-diluents do not contribute to predicting frozen-thawed semen fertility or the efficiency of equine AI.
OBJECTIVE: The aim was to evaluate the efficiency of post-diluents on frozen-thawed semen viability of two stallions (S1-S2).
MATERIALS AND METHODS: The cryopreserved semen was thawed at 50°C for 40 seconds. Semen motility and acrosin activity (AA) were determined during the thermo-resistance test (TRT).
RESULTS: Progressive motility of S2 semen decreased after 60 and 90 minutes of TRT (TRT60 and TRT90) on the control compared to both post-diluents. The total motility of both S1 and S2 decreased on TRT60 and TRT90 semen control versus both Ringer and Merk post-diluents. The AA on S1 was higher than S2 throughout the TRT. Pregnancy rates after artificial insemination (AI) were similar among post-diluents and stallions.
CONCLUSION: Post-diluents do not contribute to predicting frozen-thawed semen fertility or the efficiency of equine AI.