Recent advances and prospects in germplasm preservation of rare and endangered species.

Fertility preservation strategies using cryopreservation have enormous potential for helping sustain and protect rare and endangered species, especially to assist managing or 'rescuing' the genomes of genetically valuable individuals. However, wide-scale applications are still limited by significant physiological variations among species and a sheer lack of fundamental knowledge about basic reproductive traits as well as in germplasm cryobiology. Cryo-studies have been conducted in more species (mainly vertebrates) in the recent years but a vast majority still remains un-studied. Semen cryopreservation represents the most extensive effort with live births reported in more and more species after artificial insemination. Oocyte freezing remains challenging and unsuccessful in wild species and will require more research before becoming a standard procedure. As an alternative to fully grown gametes, gonadal tissue preservation has become a promising option in vertebrates. Yet, more fertility preservation options are necessary to save species so a change in strategy might be required. It is worthwhile thinking beyond systematic characterizations and considering the application of cutting edge approaches to universally preserve the fertility of a vast array of species.