Fertility and sterility · 2001
Effect of pretreatment of semen with pokeweed antiviral protein on pregnancy outcome in the rabbit model.
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D'Cruz O J, Uckun F M
Аннотация
OBJECTIVE: To determine whether artificial insemination of semen pretreated with pokeweed antiviral protein, a 29-kD antihuman immunodeficiency virus (HIV) protein purified from the leaves of Phytolacca americana, has any adverse effects on pregnancy outcome in the rabbit model.
DESIGN: Prospective, controlled study.
SETTING: Center for Advanced Preclinical Sciences at the Parker Hughes Institute.
ANIMAL(S): Forty-eight female and 12 male New Zealand White rabbits.
INTERVENTION(S): Fresh pooled semen obtained from 12 bucks was treated for 1 hour with and without 100 microg/mL or 1000 microg/mL pokeweed antiviral protein. Ovulated does in groups of 16 were artificially inseminated with control and pokeweed antiviral protein-treated semen and allowed to complete term pregnancy.
MAIN OUTCOME MEASURE(S): Proportion of does that became pregnant and delivered newborn rabbits; the litter size, weight, growth, and viability of pups until lactation day 5.
RESULT(S): Pokeweed antiviral protein treatment of semen had no adverse effect on gestation length, pregnancy rate, perinatal outcome, growth, and development of the offspring.
CONCLUSION(S): Pokeweed antiviral protein shows clinical potential as a safe, prophylactic antiviral agent in assisted reproduction in HIV-1 discordant couples.
DESIGN: Prospective, controlled study.
SETTING: Center for Advanced Preclinical Sciences at the Parker Hughes Institute.
ANIMAL(S): Forty-eight female and 12 male New Zealand White rabbits.
INTERVENTION(S): Fresh pooled semen obtained from 12 bucks was treated for 1 hour with and without 100 microg/mL or 1000 microg/mL pokeweed antiviral protein. Ovulated does in groups of 16 were artificially inseminated with control and pokeweed antiviral protein-treated semen and allowed to complete term pregnancy.
MAIN OUTCOME MEASURE(S): Proportion of does that became pregnant and delivered newborn rabbits; the litter size, weight, growth, and viability of pups until lactation day 5.
RESULT(S): Pokeweed antiviral protein treatment of semen had no adverse effect on gestation length, pregnancy rate, perinatal outcome, growth, and development of the offspring.
CONCLUSION(S): Pokeweed antiviral protein shows clinical potential as a safe, prophylactic antiviral agent in assisted reproduction in HIV-1 discordant couples.