Fertility and sterility · 1995
Effect of peritoneal fluid, follicular fluid, and their volumetric mixture on acrosomal reactivity in vitro.
Клиническое исследованиеСтатьяРандомизированное исследованиеПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Revelli A, La Sala G B, Gallicchio D, Modotti M, Piffaretti-Yanez A, Massobrio M, Balerna M
Аннотация
OBJECTIVE: To determine the effects of peritoneal and follicular fluids (PFs, FFs) on sperm acrosomal reaction (AR).
DESIGN: Prospective, randomized study.
SETTING: Three hospital-based infertility units.
PATIENTS: Twenty-three women participating in GIFT programs; 23 men participating in AIH programs.
INTERVENTIONS: Hormonal stimulation after buserelin desensitation; laparoscopy 36 hrs after hCG injection.
MAIN OUTCOME MEASURE: Percentage of acrosomally-reacted sperm.
RESULTS: Compared with Earle's medium (control), moderate but significant increases of ARs were observed as function 1) of the relative content of FF in the incubation medium and 2) as function of time (these increases were constantly lower than those registered for the respective positive control, i.e. 2x frozen/thawed sperm). In contrast, when PF alone was present in the incubation medium, no such effects on AR were registered.
CONCLUSIONS: FF and mixtures of PF and FF--but not PF alone--seem to induce some rapid and time-dependent processes which finally lead to an AR. Therefore, and independently on the infertility cause (tubal, male-dependent, unexplained infertility), PF seems able to exert effects on sperm motility (Revelli et al., Fertil. Steril 57, 654-60 [1992]) while maintaining an unreacted sperm status.
DESIGN: Prospective, randomized study.
SETTING: Three hospital-based infertility units.
PATIENTS: Twenty-three women participating in GIFT programs; 23 men participating in AIH programs.
INTERVENTIONS: Hormonal stimulation after buserelin desensitation; laparoscopy 36 hrs after hCG injection.
MAIN OUTCOME MEASURE: Percentage of acrosomally-reacted sperm.
RESULTS: Compared with Earle's medium (control), moderate but significant increases of ARs were observed as function 1) of the relative content of FF in the incubation medium and 2) as function of time (these increases were constantly lower than those registered for the respective positive control, i.e. 2x frozen/thawed sperm). In contrast, when PF alone was present in the incubation medium, no such effects on AR were registered.
CONCLUSIONS: FF and mixtures of PF and FF--but not PF alone--seem to induce some rapid and time-dependent processes which finally lead to an AR. Therefore, and independently on the infertility cause (tubal, male-dependent, unexplained infertility), PF seems able to exert effects on sperm motility (Revelli et al., Fertil. Steril 57, 654-60 [1992]) while maintaining an unreacted sperm status.