Does Microfluidic Sperm Sorting Affect Embryo Ploidy in Human In Vitro Fertilization?

The genetic status of embryos obtained during in vitro fertilization (IVF) programs using spermatozoa selected by microfluidic sorting was evaluated. Genetic testing was conducted on 127 embryos in the main group and 194 in the control group. The embryos were cultured under standard conditions; blastocyst biopsy was performed on day 5 for subsequent preimplantation genetic testing for aneuploidies using high-throughput sequencing. The use of microfluidic chips significantly increased the yield of blastocysts (p = 0.03) without affecting fertilization rates or embryo quality. However, when assessing the genetic status of embryos, no significant differences were found: the frequency of embryos with a normal karyotype was 29.9% in the main group and 32.4% in the control group (odds ratio, OR = 0.88). Microfluidic sperm sorting improves embryological parameters, particularly blastulation rates, but has no statistically significant effect on the genetic status of preimplantation human embryos, leaving this topic open for further investigation.