An effective system to produce smoke solutions from dried plant tissue for seed germination studies.

PREMISE OF THE STUDY: An efficient and inexpensive system was developed to produce smoke solutions from plant material to research the influence of water-soluble compounds from smoke on seed germination. •

METHODS AND

RESULTS

Smoke solutions (300 mL per batch) were produced by burning small quantities (100-200 g) of dried plant material from a range of species in a bee smoker attached by a heater hose to a side-arm flask. The flask was attached to a vacuum water aspirator, to pull the smoke through the water. The entire apparatus was operated in a laboratory fume hood. •

CONCLUSIONS

Compared with other smoke solution preparation systems, the system described is easy to assemble and operate, inexpensive to build, and effective at producing smoke solutions from desired species in a small indoor space. Quantitative measurements can be made when using this system, allowing for replication of the process.