In vitro incubation of lenses: a model for investigations on lens transparency by varying the perfusion rate, osmolarity and temperature of the culture medium.

A Beckman photometer was modified so that the unfiltered beam of a tungsten lamp could pass vertically through a special chamber. This particular chamber, containing two compartments, allowed certain modifications of the incubation conditions with respect to the anterior and/or posterior lens surface. The temperature of the incubation media was variable between 15 degrees and 37 degrees C; they were led into the chamber by hose gauge at different perfusion rates. The light transmission measured by the photometer was registered by a Honeywell recorder. Preliminary results of the various incubation conditions on the lens transparency of pig lenses are presented.