Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2026
Changes in Corneal Epithelial Barrier Function and Zonula Occludens-1 Distribution with Concomitant Use of Silver Nitrate and Latanoprost in Rabbits.
СтатьяНазвание и аннотация: Перевод в очереди
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Fukuda Masamichi, Sasaki Yu, Kiyoi Takeshi, Ishida Hidetoshi, Sasaki Makoto, Masuoka Takayoshi, Kubo Eri, Sasaki Hiroshi
Аннотация
PURPOSE: This study investigated the effects of silver nitrate (AgNO3), a preservative in ophthalmic solution, alone and in combination with latanoprost (LP), on corneal epithelial barrier function and zonula occludens-1 (ZO-1) distribution in the corneal epithelium of rabbits.
METHODS: Twelve adult male albino New Zealand white rabbits (weighing 3.0-3.5 kg) were the subjects in this study. These mature rabbits received five instillations at 5-min intervals of AgNO3 (0.01, 0.1, or 1%), benzalkonium chloride (BAK 0.02%), LP (0.005%) alone, or LP combined with AgNO3 (0.01 or 1%). Corneal resistance (CR) was measured, and histological analyses were performed.
RESULTS: AgNO3 1% increased CR (108.2%); however, CR significantly decreased when combined with LP (P < 0.05). Marked CR reduction was observed in the eyes administered BAK and LP + AgNO3 1%. Immunostaining showed preserved ZO-1 in the eyes that received AgNO3 1% and LP + AgNO3 0.01%, whereas those that received BAK, LP alone, and LP + AgNO3 1% demonstrated disrupted ZO-1.
CONCLUSIONS: A high concentration of AgNO3 with LP exacerbated corneal epithelial barrier impairment, which highlights the importance of preservative concentration control in ophthalmic formulations.
METHODS: Twelve adult male albino New Zealand white rabbits (weighing 3.0-3.5 kg) were the subjects in this study. These mature rabbits received five instillations at 5-min intervals of AgNO3 (0.01, 0.1, or 1%), benzalkonium chloride (BAK 0.02%), LP (0.005%) alone, or LP combined with AgNO3 (0.01 or 1%). Corneal resistance (CR) was measured, and histological analyses were performed.
RESULTS: AgNO3 1% increased CR (108.2%); however, CR significantly decreased when combined with LP (P < 0.05). Marked CR reduction was observed in the eyes administered BAK and LP + AgNO3 1%. Immunostaining showed preserved ZO-1 in the eyes that received AgNO3 1% and LP + AgNO3 0.01%, whereas those that received BAK, LP alone, and LP + AgNO3 1% demonstrated disrupted ZO-1.
CONCLUSIONS: A high concentration of AgNO3 with LP exacerbated corneal epithelial barrier impairment, which highlights the importance of preservative concentration control in ophthalmic formulations.